Animal Use Guidelines & Policies
- Expired IACUC Protocol Animal Maintenance
- Policy followed by IACUC and RAR for animals on IACUC protocols that expire.
- Availability of Protocols
- Policy followed by IACUC and RAR requiring approved IACUC protocol(s) be
- available in the laboratory, research, or teaching area and accessible to
- personnel listed on the protocol.
- Shared Animal Use Areas
- Policy for shared surgery or procedure areas requiring single point of contact.
- IACUC Guidelines for Using Avertin® (2,2,2 Tribromoethanol)
- Policy overview
- Anesthetics, Analgesics and Tranquilizers in Lab Animals

- Drug Selection, Induction, Support & Monitoring
- Blood Collection

- Restraint Procedures, Draws - Single, Multiple, Terminal, Common sites
- Cage Space Guidelines

- Includes Mice breeding and Agricultural Animals
- Guidelines for Euthanasia of Animals Used in Research, Teaching & Display
- (formerly appendix N)
- Euthanasia

- Criteria, Approved methods, Carbon dioxide chamber
- Housing and Husbandry for Lab Animals

- Room & Cage Conditions, Food & Water
Immunization
- Adjuvants, Route of Administration
- Restraint and Handling

- By Species, Restraint devices
- IACUC Policy on Anesthesia, Surgery and Post-Procedural Records

- All animals experiencing anesthesia or surgical procedures must have a
- concurrently recorded and readily available record detailing anesthesia data,
- intra-operative monitoring, and post-procedural care.
- Surgery

- Facilities, Use of Expired Materials, Post-procedural Care, Antibiotic Guidelines
- Tail Snipping

- For Rodents less than 50 grams
- Transporting Animal
- University of Minnesota Policy for within and between buildings
- Use of Fishes

- From the American Fisheries Society, American Institute of Fishery Research
- Biologists , and American Society of Ichthyologists and Herpetologists
- Use of Live Amphibians & Reptiles

- From the American Society of Ichthyologists and Herpetologists, The
- Herpetologists' League, and Society for the Study of Amphibians and Reptiles
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Agricultural Animal Guidelines
- Blood Collection and Intravenous Techniques with Pigs

- From the Department of Biochemistry, Physiology and Nutrition, Norwegian
- School of Veterinary Science, and Norwegian Independent Meat Association
- Cage Space Guidelines

- For commonly used farm animals
- Euthanasia

- Approved Methods for agricultural animals
- Restraint and Handling

- Including goats, sheep, calves, and swine
For more agricultural procedures, the
Guide for the Care and Use of Agricultural Animals in Agricultural Research and Teaching
is for sale through the Federation of Animal Science Societies (FASS).
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Guidelines for the Prevention, Assessment and
Relief of Pain and Distress in Laboratory Animals
These guidelines are used by IACUC, RAR and by regulatory agencies to determine
if an animal is experiencing pain or distress. A key aspect of the animal welfare
regulations is that pain and distress be minimized whenever possible.
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Points of Emphasis and Tip Sheets
Hot Topics To Maintain Compliance
Protocol Amendments Versus Vet Approved Minor Changes
Animal Transport
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- Certifying a Biological Safety Cabinet
Use of Expired Drugs or Materials
Calibration of Anesthetic Vaporizers
General Safety Concerns
Maintenance of Guillotines Used for Euthanasia Decapitation
Investigator Managed Housing Area (IMHA) Oversight
Identification of Animals Use in Research or Teaching Activities
Use of Non-Pharmaceutical-Grade Compounds in Research
Personal Protective Equipment
Sharps Injury Prevention
Expired Protocols
Anesthesia, Surgery, and Post-Operative Records
Sample Recordkeeping Forms:
Keeping Research Areas Secure
Neuromuscular Blocking Agents
Use of Hypothermia as Anesthesia for Neonatal Rodents
Amphibian, Reptile, and Fish Euthanasia
Special Diet/Water Recordkeeping |